Mycoplasma hominis deep wound infection after neuromuscular scoliosis surgery: the use of real-time polymerase chain reaction (PCR)

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Application of Real-time Polymerase Chain Reaction (RT-PCR)

The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...

Survey on association between Mycoplasma hominis endocervical infection and spontaneous abortion using Polymerase Chain Reaction

BACKGROUND Mycoplasma infections are suggested as etiology of adverse pregnancy outcomes. OBJECTIVE The aim of this study was to evaluate the association of Mycoplasma hominis (M. hominis) infection and spontaneous abortion among pregnant women. MATERIALS AND METHODS In this case-control study that was conducted from August 2012 to January 2013, totally, 109 women were included with spontan...

Real-time polymerase chain reaction.

Real-time PCR is the state-of-the-art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real-time PCR machines of different design is continuously increasing. This review provides a survey of the instruments and assay formats available and discusses...

survey on association between mycoplasma hominis endocervical infection and spontaneous abortion using polymerase chain reaction

background: mycoplasma infections are suggested as etiology of adverse pregnancy outcomes. objective: the aim of this study was to evaluate the association of mycoplasma hominis (m. hominis) infection and spontaneous abortion among pregnant women. materials and methods: in this case-control study that was conducted from august 2012 to january 2013, totally, 109 women were included with spontane...